Figure 5.

TNF-α mediates SDF-1α–induced astrocyte cell death. Confluent astrocytes in 12-well plates were incubated with SDF-1α, TNF-α, or SDF-1α plus anti–TNF-α antibodies (2 μg/ml) for 3 days; or exposed to H₂O₂ (100 μM) for 30 minutes followed by incubation with normal medium for 3 days; and then an MTT activity assay was performed. Mean ± SD of MTT activity obtained from triplicate treatments is shown (Student’s t test: *SDF-1α treatment vs. untreated control, P < 0.001; **H₂O₂ treatment vs. untreated control, P < 0.001; ^#TNF-α treatment vs. untreated control, P < 0.001; ^##treatment with anti–TNF-α antibody + SDF-1α vs. SDF-1α alone, P = 0.0058). Untreated control was plotted as 100%. Data represent three experiments.