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. 1998 Jul 7;95(14):8108–8112. doi: 10.1073/pnas.95.14.8108

Figure 2.

Figure 2

Inhibition of Notch1 receptor processing in the presence of BFA or A23187 in HeLa(N1) (A) and in the presence of α1-PDX in Jurkat cells (B). Identical amounts (100 μg) of the following extracts were run on a 6.5% SDS-polyacrylamide gel and incubated after transfer with antiserum IC. (A) Extracts from HeLa(N1) cells treated (3 hr) with BFA (50 μg/ml) (lanes 3 and 4) or with A23187 (2 μM) (lanes 1 and 2) in the presence (lanes 1, 3, and 5) or absence (lanes 2, 4, and 6) of 50 μg/ml cycloheximide. (B) Extracts from Jurkat cells (lane 2) or Jurkat cells stably transfected with the α1-PDX cDNA (clone C12D, lane 1). Positions of full-length Notch (p300) and p120 processing product are indicated.