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Principle of ATP synthesis rate measurement by 31P NMR spectroscopy. (a) Control spectrum in one volunteer: the saturation pulse is applied symmetrically to the γATP Larmor frequency. (b) Spectrum with γATP saturation acquired in the same volunteer (NS = 128). Subtraction of spectrum (b) from (a) gives the fraction of Pi involved in the synthesis of ATP, as shown on the Pi peak. ppm, parts per million.
