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13C NMR data. (a) 13C spectra acquired in a volunteer before and at the end of the [2-13C]acetate infusion. (b) Time course of glutamate C4 and glutamate C2 peak areas during the same experiment. Glutamate C4 appears soon after the beginning of the [2-13C]acetate infusion. The increase in glutamate C2 is delayed in comparison to that of glutamate C4, reflecting the extra turn of the TCA cycle required to randomize the 13C-labeled carbon into this position.
