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Effect of 5-HT on intracellular pH. Ducts were superfused with the standard HCO3–-buffered solution in the presence of secretin (1 nM). (a) One micrometer 5-HT was applied to the bath. Representative of six experiments. (b) Duct cells were acid loaded by exposure to 2-minute pulses of 20 mM NH4+ in the absence and presence of 5-HT (1 μM). Representative of five experiments.
