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. 2001 Sep 1;108(5):739–747. doi: 10.1172/JCI12563

Figure 2.

Figure 2

Quantitation of inflammatory cell infiltrates in the CNS of wild-type (WT), STAT4–/– (STAT4KO), and STAT6–/– (STAT6KO) mice. Quantitation of immunohistochemically stained spinal cord sections for CD4 and CD8 T cells and macrophages from C57BL/6 wild-type mice, STAT4–/– mice, and STAT6–/– mice. Spinal cord sections from three mice from each group were harvested on day 16 (a) and day 50 (b) and stained for CD4 (white bars), CD8 (dark gray bars), macrophages (black bars), and granulocytes (light gray bars). At least three sections from different levels of the cord were evaluated. Number of cells staining positive for the given marker were counted in 10 high-power fields (×40) per section. The results for one section were totaled, and the results between sections were averaged. Error bars represent standard deviation (SD). Statistical analysis was done using the Student’s t test. Results from a, day 16, show sections from STAT4–/– mice displayed significantly less CD4+ (P = 0.05) and CD8+ cells (P = 0.0005), macrophages (P = 0.02), and granulocytes (P = 0.004) than did wild-type mice. Sections from STAT6–/– mice consistently displayed more inflammatory cells than wild-type mice (however the P value was not significant), and significantly more than STAT4–/– mice (P < 0.05). Results from b, day 50, show significantly lower numbers of CD4+ cells in the STAT4–/– group compared with wild-type (P = 0.0024). There were no significant differences in inflammatory infiltrates between the STAT6–/– and wild-type mice at day 50.

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