Figure 7.
Adoptive transfer studies. (a) Adoptive transfer of wild-type cells into TCR-αβ–/– mice. Splenocytes from wild-type mice were harvested 12 days postimmunization with MOG peptide. The cells were resuspended in PBS and wild-type splenocytes at concentrations of either 100 × 106 (open circles), 50 × 106 (filled triangles), or 25 × 106 (filled squares) were injected intraperitoneally into recipient TCR-αβ–/– mice. The recipients were immunized with MOG peptide 1 day after transfer. Five recipient mice per group were used. (b) Adoptive transfer of splenocytes from STAT4–/– or STAT6–/– mice into TCR-αβ–/– mice. Splenocytes from either wild-type STAT4–/– or STAT6–/– mice were harvested 12 days after immunization with MOG peptide. The cells were resuspended in PBS and 50 × 106 splenocytes from STAT4–/– mice (filled circles) or STAT6–/– mice (filled squares) were injected intraperitoneally into recipient TCR-αβ–/– mice. The recipients were immunized with MOG peptide 1 day after transfer. Five recipient mice per group were used.