Figure 1.

Comparison of anti-CD3, anti-Vβ3, and TT responses in cells from HIV-infected patients and a healthy control. PBMCs from two HIV-infected patients and a healthy donor were labeled with PKH tracking dye and stimulated with either anti-CD3 Ab, anti-Vβ3 Ab, or TT. Other cells were left in medium alone. After 8 days, the cells were stained with Ab’s specific for CD4, Vβ3, or CD25. Cells that stained positive for cell surface markers were selected by gating, and frequency distribution histograms were generated for PKH dye fluorescence. Movement from right to left on the above histograms is evidence for cell division. The stimulus is shown to the left of the histograms, and the cells that were gated on for analysis are shown to the right of the histograms.