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. 2001 Sep 1;108(5):765–773. doi: 10.1172/JCI12687

Figure 3.

Figure 3

Characterization of the JZ1.1 T cell clone. (a) Immunophenotype by flow cytometry. JZ1.1 was stained with mAb’s directed to CD3, CD4, CD8, CD45RA, CD45RO,CD28, HLA-DR, TCRa/β, CD95, CD86, CD11a (dashed lines) as described in Methods; subclass-matched IgG reagents served as controls (solid lines). (b) Cytokine secretion analysis by flow cytometry. JZ1.1cells were subjected to intracellular staining using fluorescent conjugated-mAb’s to IL-2, IL-4, IL-10, or IFN-γ. Data are representative of three independent experiments.