Figure 12.

Chemokine and cytokine expression in rejecting allografts. Analysis of day 7 cardiac allografts by RNase protection. (a) Use of CsA did not affect intragraft expression of Fk mRNA, though overall levels in CX₃CR1^–/– were decreased (P < 0.01) compared with CX₃CR1^+/+ recipients. (b) Compared with CX₃CR1^+/+ recipients, targeting of CX₃CR1 plus CsA resulted in significant decreases in the chemokines MIP-1α, MIP-1β, RANTES, IP-10, and MCP-1. Ltn, lymphotoxin (c) Decreased levels of IFN-γ and IL-6 in CX₃CR1^–/– mice treated with CsA. (d) Reduced expression of CCR1 and CCR5 in CX₃CR1^–/– mice treated with CsA. (Mean ± SD, n = 3/group, Mann-Whitney U test, *P < 0.05, **P < 0.01, and ***P < 0.005.)