Figure 4.

Transactivation of bicoid-Tk-luc reporter in lens and corneal cells by wild-type and mutant PITX3. After normalization to β-galactosidase activity, the data are presented as luciferase values observed in co-transfections of bicoid-Tk-luc with various PITX3 forms relative to the activity of same reporter with empty vector only (pcDNA3.1). Values observed in transient transfections into B3 lens epithelial cell line are shown in grey and corneal stromal cells in black; error bars that denote SD are indicated.