Figure 4.

VSMCs derived from female PRKO mice are hyperproliferative. Aortic VSMCs derived from female PRKO mice or their WT littermates were maintained in SFM and their rate of proliferation was assessed by ³H-thymidine uptake. Unlike the WT-derived cells, the PRKO-derived cells did not become quiescent, but rather continued to proliferate markedly for at least 48 hours (³H-thymidine uptake 10.1 ± 1.6–fold and 13.1 ± 1.8–fold greater in PRKO vs. WT cells on day 1 and day 2, respectively; n = 5 independent experiments; *P < 0.001 vs. WT for each day).