Figure 2.

The type of adjuvant can affect the outcome of ISCI. (a) Seven female Lewis rats, immunized (7 days before contusion) with spinal cord homogenate (SCH) emulsified in CFA containing 0.5 mg/ml M. butyricum, recovered significantly better (P < 0.05, two-way ANOVA with replications; *P < 0.05, **P < 0.01, two-tailed Student’s t test) than did seven control littermates injected with PBS in the same adjuvant. (b) The same immunization protocol, when applied with a stronger adjuvant, has the opposite effect. Rats immunized with spinal cord homogenate in CFA containing 5 mg/ml M. tuberculosis developed severe EAE symptoms and showed significantly worse recovery than did six control littermates injected with PBS in the same adjuvant (P < 0.05, two-way ANOVA with replications).