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. Author manuscript; available in PMC: 2007 Nov 26.
Published in final edited form as: Dev Dyn. 2007 Jan;236(1):251–261. doi: 10.1002/dvdy.21014

Fig. 3.

Fig. 3

Depletion of chordin mRNA in Xenopus embryos caused defects in pronephros and muscle formation. A: Blot hybridization of RNA isolated from uninjected control embryos (lane 1), embryos injected with increasing amounts of the control N,N-diethyl-ethylenediamine (DEED) oligonucleotide (lanes 2-4), and embryos injected with increasing amounts of the chordin DEED-antisense oligonucleotide (lanes 5-7). The blot was hybridized with probes to detect chordin and goosecoid mRNA. B-E: Morphology of chordin mRNA-depleted embryos. B: Embryos injected with chordin-antisense DEED oligo. C: Embryo injected with control DEED oligonucleotide. D: Uninjected control embryo. E: Embryo injected with chordin mRNA + chordin-antisense DEED oligo. F-H: Analysis of pronephric duct formation in chordin-depleted embryos using immunocytochemistry with the 4A6 antibody. F: Injected with chordin-antisense DEED oligo. G: Injected with control DEED oligo. H: Uninjected control. I-K: Analysis of pronephric tubule formation in chordin-depleted embryos using immunocytochemistry with the 3G8 antibody. I: Injected with chordin-antisense DEED oligo. J: Injected with control DEED oligo. K: Uninjected control. L-N: Analysis of somitic muscle formation in chordin-depleted embryos using immunocytochemistry with the 12-101 antibody. L: Uninjected control. M: Injected with control DEED oligo. N: Injected with chordin-antisense DEED oligo.