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. 2007 Sep 12;35(18):6196–6206. doi: 10.1093/nar/gkm673

Figure 2.

Figure 2.

Reconstitution of Cbf5–Pf9, L7Ae–Pf9 and Cbf5–L7Ae–Pf9 sub-complexes. (A) Coomassie blue staining of purified recombinant Cbf5 and L7Ae following PAGE. M lane contains protein standards. (B) Gel mobility shift analysis of 5′-end labelled Pf9 RNA, alone (lane 1) or with increasing amounts of Cbf5 and/or L7Ae as indicated. Complexes were separated on an 8% native gel and visualized by autoradiography.