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. 2007 Aug 28;35(18):6004–6016. doi: 10.1093/nar/gkm649

Table 1.

Different sets of primers and oligos used for assays

Figure Primer/Oligo Product
1A and B, 2 C, D, 5 B, C For-AGACAAACACCACGAGAAT Rev- CGGAGTTCAGGGTGATGAGTGTGAC SMAR1 (Predominantly ϕ1 form)
1C, 5 B, C For- CTTCCTCTCCAAAATGCCAG Rev- AGAGATGGAAGGGGGAAAGA Cyclin D1
1, 2, 5 For- TACCACTGGCATCGTGATGGACT Rev- TTTCTGCATCCTGTCGGCAAT β-actin
2 E, G For-CGGCACGAGACAAACACCA Rev-TGCAATCTGAACCACATCCGC Composite UTR
2 F For-AGACAAACACCACGAGAAT Rev -TTCATCATCATCTCGTCACGA ϕ1
2 F For-CAGCAGCCGCAGCCCACAC Rev -TTCATCATCATCTCGTCACGA ϕ17
2 A, B Amplicon from For-AGACAAACACCACGAGAAT Rev- CGGAGTTCAGGGTGATGAGTGTGAC cloned in PGEM-T Easy SMAR1 (recognizes ϕ1 form predominantly)
2I Amplicon from For-AGACAAACACCACGAGAAT Rev -TTCATCATCATCTCGTCACGA cloned in PGEM-T Easy ϕ1 UTR
2I Amplicon from For-CAGCAGCCGCAGCCCACAC Rev –TTCATCATCATCTCGTCACGA cloned in TOPO-TA ϕ17 UTR
4 D–G CGAAATTAACCCTCACTAAAGGGAACCCACGGTCGACAGAAACC SL1
4G CGAAATTAACCCTCACTAAAGGGAACCACACGGTCGACAGTGACC Mutant 1
4 G CGAAATTAACCCTCACTAAAGGGAACCACATAATCGACAGGGACC Mutant 2
5 F, G For-TGAAAATGAAAGAAGATGCAGTCG, Rev-GAAACTTGCACAGGGGTTGT Probe II Cyclin D1 promoter