1A and B, 2 C, D, 5 B, C |
For-AGACAAACACCACGAGAAT Rev- CGGAGTTCAGGGTGATGAGTGTGAC |
SMAR1 (Predominantly ϕ1 form) |
1C, 5 B, C |
For- CTTCCTCTCCAAAATGCCAG Rev- AGAGATGGAAGGGGGAAAGA |
Cyclin D1 |
1, 2, 5 |
For- TACCACTGGCATCGTGATGGACT Rev- TTTCTGCATCCTGTCGGCAAT |
β-actin |
2 E, G |
For-CGGCACGAGACAAACACCA Rev-TGCAATCTGAACCACATCCGC |
Composite UTR |
2 F |
For-AGACAAACACCACGAGAAT Rev -TTCATCATCATCTCGTCACGA |
ϕ1 |
2 F |
For-CAGCAGCCGCAGCCCACAC Rev -TTCATCATCATCTCGTCACGA |
ϕ17 |
2 A, B |
Amplicon from For-AGACAAACACCACGAGAAT Rev- CGGAGTTCAGGGTGATGAGTGTGAC cloned in PGEM-T Easy |
SMAR1 (recognizes ϕ1 form predominantly) |
2I |
Amplicon from For-AGACAAACACCACGAGAAT Rev -TTCATCATCATCTCGTCACGA cloned in PGEM-T Easy |
ϕ1 UTR |
2I |
Amplicon from For-CAGCAGCCGCAGCCCACAC Rev –TTCATCATCATCTCGTCACGA cloned in TOPO-TA |
ϕ17 UTR |
4 D–G |
CGAAATTAACCCTCACTAAAGGGAACCCACGGTCGACAGAAACC |
SL1 |
4G |
CGAAATTAACCCTCACTAAAGGGAACCACACGGTCGACAGTGACC |
Mutant 1 |
4 G |
CGAAATTAACCCTCACTAAAGGGAACCACATAATCGACAGGGACC |
Mutant 2 |
5 F, G |
For-TGAAAATGAAAGAAGATGCAGTCG, Rev-GAAACTTGCACAGGGGTTGT |
Probe II Cyclin D1 promoter |