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. 2007 Sep 18;35(18):e121. doi: 10.1093/nar/gkm682

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — (A) Different combinations of Globin-renilla RNAs (+/+), (+/−), (−/+) or (−/−) were translated at 0.1 mg/ml for 0, 15, 30 or 60 min in the nuclease-treated RRL (left panel) or untreated RRL (right panel) as indicated on the figure. Renilla luciferase activity was then determined and normalized as described in Materials and Methods section.(B) Globin-renilla RNAs (+/+), (+/−), (−/+) or (−/−) at 0.1 mg/ml were translated in both nuclease-treated RRL (left panel) or untreated RRL (right panel) in the presence of 0, 0.25, 0.5 or 1 mM added MgCl₂. The resulting translation products were resolved by 15% SDS–PAGE and autoradiography and the position of the protein products are indicated by arrows on the figure. (C) Globin-renilla RNAs (+/+), (+/−), (−/+) or (−/−) at 0.1 mg/ml were translated in both nuclease-treated RRL (left panel) or untreated RRL (right panel) in the presence of 0, 0.25, 0.5 or 1 mM added MgCl₂. Renilla luciferase activity was then determined and normalized as described in Materials and Methods section. The results presented are representative of three independent experiments are expressed as means ± SD.