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. 2007 Sep 12;35(18):e118. doi: 10.1093/nar/gkm704

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — UTF1 expression in hESCs. (A) qRT-PCR Expression analysis of RA- (for 12 days) and DMSO-induced (for 7 days) differentiation in hESCs, normalized to that of respective undifferentiated hESC lines, with two biological replicates for each sample. (B) Schematic drawing of vectors. Full-length UTF1 was cloned into phagemid pTZ-18R yielding pTZ-UTF1. Its coding region was subsequently replaced with enhanced green florescent protein (EGFP) or neomycin (Neo), yielding pTZ-UTF1-EGFP and pTZ-UTF1-Neo, respectively. Red text indicates alterations in pTZ-UTF1-EGFP. (C) Flow cytometry of UTF1-driven EGFP expression in various cell lines following transient transfection of pTZ-UTF1-EGFP. Transfection efficiencies were normalized to those determined in parallel using pCMV-EGFP and are the average of three biological replicates. See Supplementary Figure S7 for representative dot plots.