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. 2007 Sep 7;35(18):6170–6180. doi: 10.1093/nar/gkm669

Figure 3.

Figure 3.

Relaxation activity of HT, HT▵(191–206), HT(Trp205Gly) and HT(207–765) on positively or negatively supercoiled plasmid at distributive conditions. Relaxation activities of HT (A), HT(Trp205Gly) (B), HT▵(191–206) (C) or HT(207–765) (D) were assayed at distributive conditions by incubating 300 ng negatively (left panels) or positively (right panels) supercoiled pBR322 plasmid with each of the four enzymes at 37°C in an molar enzyme:plasmid ratio of 1:10. Except for these alterations, the experiment was carried out as relaxation under processive conditions shown in Figure 2. Right panel is a graphical depiction of the results, where relaxation activity is calculated as the relative loss of supercoiled substrate left after incubation and plotted as a function of incubation time. Filled squares: represent the relaxation of positive supercoils, open triangles: represent relaxation of negative supercoils. SC, supercoiled pBR322. R, relaxed pBR322. L, linearized pBR322.