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. 2007 Sep 7;35(18):6137–6149. doi: 10.1093/nar/gkm656

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — KLF4 interacted with Tip60 by coimmunoprecipitation assay. (A) Endogenous KLF4 interacted with endogenous Tip60. Total proteins were immunoprecipitated with control IgG and anti-KLF4 and anti-Tip60 antibodies. The immunoprecipitates were then probed with anti-Tip60 and anti-KLF4 antibodies. Total proteins were loaded as a control (B). KLF4 interacted with Tip60 in vitro. N-terminally HA-tagged KLF4 expression plasmid (pHA-KLF4) and C-terminally FLAG-tagged Tip60 plasmid (pFLAG-Tip60) with the respective vectors were cotransfected in combination into AGS cells. Total protein extracts were prepared 48 h after transfection. Anti-HA antibody or Anti-FLAG antibody conjugated agarose beads were added to protein extracts. After washing, immunoprecipitated proteins were separated on SDS-PAGE gel and then probed with different antibodies to detect interaction between KLF4 and Tip60.