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. 2007 Sep 7;35(18):6137–6149. doi: 10.1093/nar/gkm656

Table 1.

Positive clones from yeast two-hybrid screening using two fragments of KLF4 as the baits

Bait Blast search results from 20 positive colonies (frequency of more than 1 is indicated in parentheses) Coding region characteristic

KLF4A (1–170 aa) JunD(2), IGKC, Small membrane protein 1, macroglobulin α2, SFTPA2, human upstream binding transcription factor In-frame

c-Jun(2), prothymosin, FKBP 1A, ZFP36, MGAT1, galectin 1, CALM3,DC class II histocompatibility antigen α-chain Not in-frame

KLF4B (170–388 aa) Tip60(3), FIM protein, BDR-1, Ubc protein 38197157, PCDH16 In-frame

c-Jun (4), LDLR, SVAP1,DUSP4, APBA3 Not in-frame

Note: in-frame indicates that amino acid coding results of DNA sequence of positive clones are within the same open reading frame of GAL4 activation domain in the cloning vector. Not in-frame is the opposite.