Table 1.
Bait | Blast search results from 20 positive colonies (frequency of more than 1 is indicated in parentheses) | Coding region characteristic |
KLF4A (1–170 aa) | JunD(2), IGKC, Small membrane protein 1, macroglobulin α2, SFTPA2, human upstream binding transcription factor | In-frame |
c-Jun(2), prothymosin, FKBP 1A, ZFP36, MGAT1, galectin 1, CALM3,DC class II histocompatibility antigen α-chain | Not in-frame | |
KLF4B (170–388 aa) | Tip60(3), FIM protein, BDR-1, Ubc protein 38197157, PCDH16 | In-frame |
c-Jun (4), LDLR, SVAP1,DUSP4, APBA3 | Not in-frame |
Note: in-frame indicates that amino acid coding results of DNA sequence of positive clones are within the same open reading frame of GAL4 activation domain in the cloning vector. Not in-frame is the opposite.