Figure 2.

Increased RAG cutting on the acetylated nucleosome arrays. RAG cutting was performed on the acetylated (Acet) and non-acetylated (Non-Acet) nucleosome arrays either in the presence of Drosophila extract (Total) or following removal of proteins (Sarkosyl-stripped). Verification that the PCR reactions were in the semi-quantitative range (for each set of RAG cut templates) was achieved by confirming that the signal increased proportionately to the amount of input DNA (Supplementary Figure 2). The DNA content panel is a control PCR and shows that an equivalent amount of DNA was used per reaction. The stimulation in RAG cutting was normalized to the amount of DNA present. This stimulation of RAG cutting on the acetylated templates compares favourably with the acetylation-mediated increase in transcription (of ∼5-fold) reported previously with similarly reconstituted templates (27).