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. 2007 Sep 18;35(18):6311–6321. doi: 10.1093/nar/gkm650

Figure 5.

Figure 5.

Increased accessibility of acetylated arrays. Time course of Pvu I digestion on acetylated (Acet) and non-acetylated (Non-Acet) arrays. The percent parental band cut was calculated following quantification with a phosphorimager. The increased cutting on acetylated arrays was observed in seven independent experiments and with three different preparations of (normalized) acetylated and non-acetylated histones. The right panels show Pvu I cutting when most ATP-dependent nucleosome remodelling complex activity was inhibited by removal of ATP with apyrase. Similar experiments in which remodelling activity is inhibited by addition of a large excess of AMP-PNP also showed increased Pvu I digestion on the acetylated chromatin (data not shown). We suggest that the difference in the level of accessibility between acetylated and non-acetylated chromatin measured here is less than the observed difference in the level of RAG cutting because Pvu I (28 kDa) is much smaller than ACF (600 kDa). Therefore, Pvu I would be expected to access compacted non-acetylated chromatin more readily thereby resulting in a smaller difference in cutting compared to acetylated chromatin.