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. 2007 Sep 1;35(18):6094–6102. doi: 10.1093/nar/gkm534

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Co-purification of LysRS, LeuRS, ProRS and EF-1α. M. thermautotrophicus cell-free extracts, initially applied to a Sephacryl S300 gel filtration column, were applied to a Q-Sepharose column and extensively washed prior to development with a KCl gradient. Aminoacyl-tRNA synthesis activities were monitored in the eluted fractions; Lys-tRNA^Lys (open square), Leu-tRNA^Leu (open triangle), Pro-tRNA^Pro (filled triangle) and Ala-tRNA^Ala (filled square). The presence of EF-1α was assayed in each eluted fraction by immunoblotting with antibodies against EF-1α.