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Effects of the association of EF-1α and the archaeal MSC on the aminoacylation activities of LysRS and ProRS. Aminoacylation by A, 100 nM LysRS (filled triangle) and (B), 80 nM ProRS (filled diamond) alone or in the presence of 3.5 µM EF-1α·GTP (filled circle) or EF-1α·GDP (open circle). Inverted filled triangle EF-1α alone.
