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. 2007 Sep 5;35(18):6124–6136. doi: 10.1093/nar/gkm628

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Stimulation of P activation by Bag-1S plus Hsp90/Hop. P protein–Dε complexes were reconstituted from GrpDP (10 nM) with only Hsc70 and Hsp40 (filled squares), or in the additional presence of Hsp90 and Hop (open triangles), and supplemented with the indicated amounts of Bag-1S. Complex formation was monitored by priming assays. Signals were quantified by phosphorimaging, normalized to the signal obtained in the presence of Hsc70 and Hsp40 only, and plotted against the concentration of Bag-1S. Relative priming activities are given as means ± SD from at least four independent experiments.