Table 1.
Primer sequences and their predicted product sizes
| Primers | Forward (5′ to 3′) | Reverse (5′ to 3′) | Product size (bp) | Reference |
|---|---|---|---|---|
| RBD-1 | GGACGCAGAACAGATCAATACCGA | TCTTCAAACCACTGTCAACTCCTG | 225 | (16) |
| RBD-2 | TTAATTTGGTTTGTTTTGTGCAT | CATGCCTGACCAAAGGAGGCGTA | 132 | (16) |
| Defb3 | CTCTTCTCATTTCTCCTGGTGCT | GTGCCACAACTGCCAATCTCT | 150 | (18) |
| Defb5 | CTCTTCTCATTTCTCCTGGTGCT | TCTCACTTTACCCAGTCCACAA | 164 | (18) |
| Defb36 | AGGCCCATCATGAAGCTCCTGC | TTCCGGCTTCCGGGCAGTTAG | 227 | (18) |
| TLR-2 | GCACTTGAGCGAGTCTGCTTTC | GAACAAATAGAACTGGGGGATGTG | 361 | |
| TLR-3 | GCTTCTCACCCCAACATTGACG | ACCCTCCAACAAGTCCTCGTTC | 564 | |
| TLR-4 | GGCTGTGGAGACAAAAATGACCTC | AGGCTTGGGCTTGAATGGAGTC | 272 | |
| TLR-5-1 | TCAACACGACTGAGAGGCTCCTAC | CCCTGAAAAGCGTCTGGATTC | 217 | |
| TLR-5-2 | TCACCCTATTCGGTTCTCCTGC | CGTTCTGTGCCCATTCAAAGTC | 333 | |
| TLR-6-1 | GAGAACCCATCGTGGAGAGTTTC | GGATTTTGTGCTTGGTGACAGG | 155 | |
| TLR-6-2 | GCATCAAGAGAAGTGGTGGAGG | ACTGCCATAGCATCCTGAGATACC | 223 | |
| TLR-7-1 | CCAAGCATCTCTCCAGACTCCTTC | TGGCAAAATGGTGGGGACAG | 441 | |
| TLR-7-2 | CGACTCTCTCCTTAGCCAAAAATGG | AACCCACCAGACAAACCACACAGC | 362 | |
| TLR-9-1 | GGACGGGAACTGCTACTACAAGAAC | AGGTGGCTCAGGTGATGGAAAG | 336 | |
| TLR-9-2 | AGCCTGATTCATCTGGACCTGTC | ACTGCCACACTTCACACCGTTAG | 492 | |
| GAPDH | AGACAGCCGCATCTTCTTGT | CTTGCCGTGGGTAGAGTCAT | 207 |
GAPDH, glyceraldehyde-3-phosphate dehydrogenase; RBD, rat β-defensin; TLR, Toll-like receptor.