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. Author manuscript; available in PMC: 2007 Nov 26.
Published in final edited form as: J Hepatol. 2007 Jun 8;47(4):571–579. doi: 10.1016/j.jhep.2007.04.019

Figure 1. Effect of feeding MCDD on TLR-4 signaling molecules.

Figure 1

(A) For assessment of TLR-4, MD2 and CD14 expression, livers were collected from male C57BL/6 mice fed CD or MCDD for 3 weeks. Pre-developed assays for real-time PCR were used according to the manufacturer’s instructions (Applied Biosystems). Expression of each target mRNA was calculated relative to average values in the control group using a comparative CT method and presented as mean ± SEM of at least 4 observations/group. (B) Western blot analysis of hepatic TLR-4 expression relative to β-actin was used to confirm enhanced protein levels. Values are mean ± SEM of at least 4 observations per group. *p< 0.05, **p< 0.005 compared to control using Student’s t test.