Figure 7. Hepatic expression of (A) TNF-α, (B) TGF-β and (C) Collagen.
Livers were collected from male C57BL/6 mice fed control (CD) or methionine/choline deficient (MCDD) for 3 weeks. In addition, mice were administered clodronate liposomes (200 µg/mouse) to destroy Kupffer cells or PBS (phosphate-buffered saline) as a vehicle control as described in Methods. Pre-developed assays for real-time PCR were used to assess target mRNA levels. Statistical comparisons were made using two-way ANOVA (n= 5/group). *p< 0.05 compared to mice fed control diet; #diet-matched PBS (phosphate-buffered saline) controls.