Figure 2.

Hematoxylin and eosin–stained (HE-stained) sections of mouse vein grafts. Under anesthesia, vena cava veins of PKCδ^+/+ (a and e) or PKCδ^–/– (b, c, and f) mice were removed from the donor and isografted into carotid arteries of the recipient. Animals were sacrificed 8 weeks after surgery, and the grafted tissue fragments were fixed in 4% phosphate-buffered (pH 7.2) formaldehyde, embedded in paraffin, sectioned, and stained with HE. Arrows indicate neointima. (d) Statistical data of neointimal area measured as described in Methods. Data are means ± SEM. *Significant difference between PKCδ^–/– and PKCδ^+/+ groups, P < 0.01.