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. 2001 Nov 15;108(10):1523–1531. doi: 10.1172/JCI13787

Figure 2.

Figure 2

Functional characterization of WHVX mutants in vitro. (a) Transactivation of WHVX mutants. The reporter plasmid RSV-Luc and WHVX mutants were cotransfected at a ratio of 1:5 with a total DNA of 0.6 μg per well in a six-well plate. Luciferase activities were assayed 3 days later. Data shown are the mean values of triplicates. (b) Transcription of WHV8X mutants. Three days after transfection, HuH7 cells were harvested, and total RNAs were isolated and subjected to Northern blot analysis. The ribosomal RNAs (bottom) were shown for RNA-loading control (probing for β-actin showed similar results). (c) Replication of WHV8X mutants. Three days after transfection, WHVX-transfected HuH7 cells were harvested, and the core particle–associated WHV DNA were isolated and subjected to Southern blot analysis. Lanes are as indicated. SS, single-stranded WHV DNA. All the results are representative of at least three independent experiments.