Figure 2 Western blot analysis of factor VII activating protease (FSAP) protein in plasma, bronchoalveolar lavage (BAL) fluid and lung tissue. Western blotting with FSAP specific antibodies was performed to characterise FSAP protein in (A) plasma and (B) BAL fluid of healthy controls and of patients with extrapulmonary acute respiratory distress syndrome (ARDS) without pulmonary infection (ARDS), ARDS with primary lung infection (ARDS + Pneu) and with cardiogenic pulmonary oedema (CLE) as indicated. Representative patients for each entity are shown (healthy controls: 2/15, CLE: 2/5, ARDS: 3/15; ARDS + Pneu: 2/8). The inset shows the presence of the 29 kDa light chain of FSAP in concentrated BAL fluid from patients with ARDS but not from controls (healthy controls: 3/3, ARDS: 3/3). (C) Characterisation of FSAP protein in lung homogenate of patients with ARDS and controls (shown: myocardial infarction). Representative patients for each entity are shown (controls: 3/5, ARDS: 3/3; ARDS + Pneu: 3/4). scFSAP, single chain FSAP; hcFSAP, heavy chain of proteolytically active two chain FSAP; lcFSAP, light chain of proteolytically active two chain FSAP.