Figure 9 Uptake of factor VII activating protease (FSAP) by alveolar macrophages. (A) After incubation of mouse alveolar macrophages with human FSAP in the absence (left panel) or the presence of chloroquine (right panel) for 10, 30 and 60 min, respectively, cell extracts were analysed by western blotting using an antibody specific for human FSAP. In parallel, cell extracts were analysed in the absence of FSAP (control = Co); hcFSAP, heavy chain of proteolytically active two chain FSAP; lcFSAP, light chain of proteolytically active two chain FSAP. (B) At the indicated time points, cells were stained for human FSAP (left panels) or for lysosomes (middle panels) and an overlay of both images was generated in each case (right panels). Bar = 5 μm. One representative experiment out of five independent experiments for each time point is shown. The insets show high power images of single macrophages.