Figure 2 Inhibition of histamine (100 µM) induced gel contraction by (A) the phospholipase C (PLC) inhibitor U73122 (10 μM; n = 3 different patients), (B) the myosin light chain kinase (MLCK) inhibitor ML‐7 (30 μM; n = 6), and (C) the Rho‐associated coiled coil forming kinase (ROCK) inhibitor Y27632 (n = 5 for histamine alone and 10 μM Y27632, n = 4 for 3 μM Y27632). Contraction curves to histamine with inhibitory treatments (U73122, ML‐7, 3 μM and 10 μM Y27632) were all significantly different from that of histamine alone (p⩽0.05, repeated measures two‐way ANOVA).