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. 2001 Nov 1;108(9):1341–1348. doi: 10.1172/JCI11235

Figure 4.

Figure 4

Effect of genes that alter mitochondrial superoxide production on eNOS activity in BAECs. Cells were incubated in 5 mM or 30 mM glucose alone, or in 30 mM glucose plus either UCP-1– or MnSOD-expressing adenoviral vectors, or control vector. Cells expressing UCP-1 and MnSOD were also incubated in 30 mM glucose supplemented with 5 mM glucosamine. eNOS activity was determined after immunoprecipitation as described in Methods. *P < 0.01 compared with cells incubated in 5 mM glucose. n = 5 for each group.