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. 2001 Nov 1;108(9):1341–1348. doi: 10.1172/JCI11235

Figure 6.

Figure 6

Effect of hyperglycemia, GFAT antisense oligonucleotides, and genes that alter mitochondrial superoxide production on eNOS O-linked GlcNAc and phospho-eNOS(Ser1177). Cells were incubated in 5 mM glucose alone, 5 mM glucose plus wortmannin, 30 mM glucose alone, and 30 mM glucose plus either GFAT antisense, GFAT inverse, or GFAT scrambled oligonucleotides; or 30 mM glucose plus UCP-1– or MnSOD-expressing adenoviral vectors, as indicated. (a) Representative IP-Western blot. (b) eNOS O-linked GlcNAc blotted with RL2 antibody (relative densitometric means of three IP-Western blots). (c) Phospho-eNOS(Ser1177) (relative densitometric means of three IP-Western blots). *P < 0.01 compared with cells incubated in 5 mM glucose.