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. 2001 Nov 1;108(9):1341–1348. doi: 10.1172/JCI11235

Figure 7.

Figure 7

Effect of hyperglycemia on O-linked GlcNAc and phospho-eNOS(Ser1177) in myc-tagged wild-type human eNOS and myc-tagged human eNOS mutated at the Akt site. Cells were transfected as described in Methods and incubated in either 5 mM glucose or 30 mM glucose. Western blots were performed for O-linked GlcNAc and phospho-eNOS(Ser1177) and normalized by blotting for myc. (a) Representative IP-Western blot. (b) eNOS O-linked GlcNAc/myc expressed as percent of wild-type eNOS in 5 mM glucose (relative densitometric means of three IP-Western blots). (c) Phospho-eNOS(Ser1177)/myc expressed as percent of wild-type eNOS in 5 mM glucose (relative densitometric means of three IP-Western blots). *P < 0.01 compared with wild-type transfected cells incubated in 5 mM glucose.