Figure 1.

Flow cytometry confirmation of the absence or presence of CD4⁺ and/or CD8⁺ T cells. (a) CD4⁺ and CD8⁺ T cells are absent from spleens obtained from nu/nu mice. (b) After nu/nu mice were adoptively transferred with a T cell population from a wild-type control mouse, both CD4⁺ and CD8⁺ T cells were detected in spleens. The reconstitution of these cells in nu/nu mice ranged between 2 and 6%. (c) CD4-deficient mice show an absence of CD4⁺ T cells, but a normal complement of CD8⁺ T cells when compared with a wild-type control (not shown). (d) CD4-reconstituted CD4-deficient mice show a CD4⁺ population of approximately 2%.