Figure 12.

Flow cytometry confirmation of adoptive transfer of CD28-deficient and IFN-γ–deficient CD4⁺ T cells into T cell–deficient mice. (a) Wild-type spleen showing normal numbers of cells stained positively for CD4 (15.8%) and CD8 (13.1%). (b) nu/nu mice were reconstituted with CD4⁺ T cells (5.5% positive stained) from mice deficient in CD28. (c) nu/nu mice were reconstituted with CD4⁺ T cells (4% positive stained) from mice deficient in IFN-γ.