Figure 2.

DDR2 is induced in stellate cells during liver injury. (a) Northern blot analysis of DDR2 mRNA expression in rat stellate cells isolated after administration of a single dose of CCl₄ or after bile duct ligation. (b) Summary histogram demonstrating the induction of DDR2 during liver injury. Results are expressed in the histograms as the ratio of DDR2 mRNA versus GAPDH mRNA. (c) Total liver extracts from rats treated 4 weeks with CCl₄ or corn oil vehicle alone were lysed in RIPA buffer. DDR2 was immunoprecipitated with the R2-JM Ab, followed by Western blot analysis with an anti-phosphotyrosine Ab (4G10) or the anti-DDR2 Ab R2-JM.