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Type I collagen stimulates DDR2 phosphorylation in stellate cells. HSC-T6 stellate cells were stimulated with 10 μg/ml type I collagen for increasing intervals and lysed in RIPA buffer. DDR2 was immunoprecipitated with the anti-DDR2 Ab R2-JM, followed by Western blot analysis with an antiphosphotyrosine Ab (4G10) or anti-DDR2 (R2-JM).
