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DDR2 promotes MMP-2 expression and activity in stellate cells. Serum-free supernatants from HSC-T6 cells expressing a kinase-dead mutant of the receptor (kd-DDR2), GFP alone (control), or the constitutively active Fc-DDR2 were analyzed in triplicate experiments for (a) MMP-2 protein using Western blots and (b) MMP-2 activity by gelatin zymography. Relative expression was quantitated by the NIH Image software and shown beneath each lane.
