Figure 8.

Increased TIMP-2 or the MMP inhibitor GM6001 are required to inhibit proliferation of stellate cells overexpressing DDR2. (a) HSC-T6 cells expressing GFP alone (control) or the constitutively active Fc-DDR2 were cultured for 48 hours in DMEM with 1% FCS in the presence of recombinant TIMP-2 or GM6001, 10 μM (GM). Incorporation of [³H]-thymidine during the last 6 hours of the experiment was measured. (b) TIMP-2 and MT1-MMP expression were analyzed by Western blots in the serum-free supernatants, or in the lysates from HSC-T6 cells expressing GFP alone (control), or Fc-DDR2.