Figure 1.

PCR amplification of ABCA1 gene from WT and ABC1^–/– peritoneal macrophages. Elicited peritoneal macrophages were obtained from irradiated mice transplanted with donor bone marrow (donor bone marrow→recipient mice). Total DNA was isolated and subjected to PCR amplification using specific primers for WT ABCA1 (a) or the disrupted ABCA1 (b) gene. RNase protection assay for ABCA1 and cyclophilin was performed on 10 μg total RNA obtained from the liver and spleen of recipient mice (c). Protected ABCA1 fragments were visualized by autoradiography and quantitated by a phosphorimager. Similar levels of cyclophilin were seen in all samples.