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Rum1 stabilization is required for re-replication of the genome in Δsud1 cells but does not cause the defect in rapid proteolysis of Cdc18. (A) Degradation of Cdc18 in wild-type and Δsud1 Δrum1 strains was measured as described above (see Materials and Methods). (B) Wild-type, Δsud1, and Δsud1 Δrum1 strains were grown overnight in minimal medium containing low nitrogen to induce re-replication. DNA content was analyzed by flow cytometry.
