Figure 4.

Sud1 is physically associated with the ubiquitin pathway in fission yeast. (Left) Extracts containing HA-tagged Sud1 protein were immunoprecipitated with either control nonimmune rabbit serum (lane 2) or anti-ubiquitin antibodies (lane 3), separated by SDS/PAGE, and immunoblotted with anti-HA mAb 12CA5. One-tenth of the original extract was loaded on the same gel for comparison. (Right) Extracts containing HA-tagged Sud1 were immunoprecipitated with either control nonimmune mAb (lane 4) or HA-specific mAb 12CA5 (lane 5), and then immunoblotted with anti-ubiquitin antibodies. The position of high molecular weight protein-multiubiquitin adducts is indicated.