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. 1998 Jul 7;95(14):8159–8164. doi: 10.1073/pnas.95.14.8159

Figure 5.

Figure 5

Sud1 and Cdc18 bind one another in a phosphorylation-dependent manner. (A) Extracts derived from cells expressing HA epitope-tagged Sud1 and MYC epitope-tagged Cdc18 were immunoprecipitated with either control nonimmune mAb (lanes 2 and 6), anti-HA mAb 12CA5 (lanes 3 and 7), or anti-MYC mAb 9E10 (lanes 4 and 8). Immunoprecipitates and an aliquot of the input extract (lanes 1 and 5) were separated by SDS/PAGE and immunoblotted with either 9E10-horseradish peroxidase conjugate (9E10-HRP; Upper) or 12CA5 mAb (Lower) Ig, immunoglobulin. (B) HA-Sud1 was expressed together with either wild-type MYC-Cdc18 or MYC-Cdc18ΔCDK1–6. Extracts were immunoprecipitated with control nonimmune mAb (lanes 2 and 5) or 12CA5 mAb (lanes 3 and 6), then immunoblotted with 9E10-horseradish peroxidase conjugate (Upper) and 12CA5 mAb (Lower). Aliquots of the input extracts (lanes 1 and 4) were included on the same gel for comparison.