Figure 1.

Binding of AGEs to NRK 52E cell membranes. Unlabeled AGE-BSA (filled squares) competed with ¹²⁵I AGE-BSA for membrane binding; unglycated BSA (filled triangles) did not. Shown is one representative of five independent experiments. Maximal binding was defined as binding in the absence of competition and was 4.27 ± 0.4% of applied radioactivity. Nonspecific binding was defined as binding in the presence of maximal competition (10^–4 M AGE-BSA) and was 7.7 ± 0.6% of maximal binding. B/Bo(%), % specific binding.