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Construction of MIP-1α antisense. The first exon of MIP-1α cDNA was generated by standard PCR techniques as described in Methods. The identity of the MIP-1α cDNA was confirmed by sequence analysis, and it was subcloned into the pcDNA3 vector. Clones that had the reverse orientation for the MIP-1α cDNA were screened by PCR, and the orientation was confirmed by DNA sequence analysis.
