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. 2007 Dec 5;2(12):e1257. doi: 10.1371/journal.pone.0001257

Figure 4. Early S phase recruitment of MRE11 and NBS1 to DS.

Figure 4

A) Mutu I cells were fractionated by centrifugal elutriation and analyzed for cell cycle synchronization by FACS. Cell cycle fraction numbers correspond to rates of counter flow used for centrifugal elutriation. B) MutuI cells were fractionated by centrifugal elutriation as shown in panel B, and then analyzed by chromatin immunoprecipitation with antibodies to MRE11, NBS1, and control IgG. Immunoprecipitated DNA was quantified by real-time PCR with primers specific for OriP or control OriR regions of the EBV genome. ChIP values were presented as percentage of input DNA. Cell cycle fractions correspond to the FACS analysis in panel A.